PlantScreenBabban dandalin nazarin tsire-tsire na daji

——Field-based High-throughput Phenotyping PlatForm

Gabatar da sauri, daidai, da kuma high-gudanarwa ba lalacewa multi-siffofin bincike ikon a kan daji girma tsire-tsire shine babban kalubale da aka fuskanci a karni na 21 gona kwayoyin halitta (Andrade-Sanchez et al. 2014, Furbank and Tester 2011, Houle et al. 2010) . Tsarin nazarin tsire-tsire na daji yana da mahimmanci ga ilimin kwayoyin halitta, fasahar halittu, kiwon amfanin gona, da binciken sa ido kan amsawar amfanin gona ga canjin yanayi, ƙasa, gudanar da noma, da sauransu, musamman aikin gona na zamani, aikin gona mai hankali.

PlantScreenTsarin binciken siffofin tsire-tsire mai yawa na daji ya haɗa fasahohin zamani kamar tsarin sarrafa kansa, nazarin auna hoton fluorescence na chlorophyll, nazarin hoton zafi na tsire-tsire, nazarin high spectrum na tsire-tsire, nazarin hoton launi na RGB da kuma dandalin babban bayanai na intanet + siffofin tsire-tsire, don cimma ma'aunin siffofin siffofin siffofin siffofin siffofin siffofin siffofin siffofin siffofin siffofin siffofin siffofin siffofin siffofin siffofin siffofin siffofin siffofin siffofin siffofin siffofin siffofen A matsayin masana'antun farko na duniya da ke samar da tsarin daukar hoto na chlorophyll fluorescence, kamfanin PSI yana da ingantaccen fasaha a duniya a fannin nazarin zane-zane na tsire-tsire, dandamali na nazarin zane-zane kamar nazarin zane-zane na chlorophyll fluorescence yana saPlantScreenZama mafi m kayan aiki kayan aiki don tsire-tsire phenotype analysis da kuma aiki imaging analysis.

Ayyuka:

1) Babban Multi-aiki na'urar firikwensin dandali, hade da chlorophyll fluorescence imaging, RGB imaging, infrared thermal imaging, LiDAR、 High spectrum imaging da sauran daban-daban ci gaba high karshen na'urorin ganewa, cikakken bincike:

a) Binciken siffofin siffofin tsari (hoton RGB da LiDAR)

b) Functional bincike (chlorophyll fluorescence imaging)

c) Binciken siffa da ci gaba (RGB imaging da LiDAR)

d) Ayyukan photosynthesis (chlorophyll fluorescence imaging)

e) Biological tilasta da kuma non-biological tilasta amsa (Chlorophyll fluorescence imaging, high spectrum imaging, infrared thermal imaging)

f) physiological muhalli yi ciki har da photosynthetic physiology, aeroporous dynamics, biochemical metabolism nuna alama da sauransu (chlorophyll fluorescence imaging, high spectrum imaging, infrared thermal imaging)

2) Manyan duniya FluorCam fluorescence imaging fasaha, shi ne muhimmanci bincike fasaha na amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da amfani da am Fita da musamman high m chlorophyll fluorescence hoto ruwan tabarau, hoto yankin har zuwa 35cm x 35cm (abokin ciniki za a iya tsara 80cm x 80cm), shi ne mafi girma fasaha kayan aiki a duniya daya chlorophyll fluorescence hoto yankin

3) Za a iya shigar da shi a kan tarakta don motsi ta atomatik imaging analysis, kuma za a iya shigar da shi a kan wani musamman ta atomatik aiki dandamali yayin da shi ke aiki ta atomatik a kan samfurin band hanya yayin da samfurin band cikakken rufi ta atomatik dubawa imaging da kuma online analysis

4) Babban dandamali na bayanai na bincike na bayanai, gami da sarrafa tsarin, tattara bayanai, nazarin sarrafa bayanai da nunawa ta kan layi, bayanan bayanai, da sauransu

5) Goyon bayan fasaha na ƙungiyar ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙ

6) Za a iya sanya PhenoUAS drone-tushen fasahar UAV-tushen dandamali na binciken bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan bayanan

7) Za a iya sanya tashar sa ido ta yanayin ƙasa don bincika dangantakar yanayin muhalli da halaye na phenotype

8) Zaɓi don tsarin sa ido kan tsire-tsire na tsire-tsire na tsire-tsire na tsire-tsire na tsire-tsire na tsire-tsire na tsire-tsire na tsire-tsire na tsire-tsire na tsire-tsire na tsire-tsire na tsire-tsire

9) Za a iya zaɓi ta atomatik auna dijital kwalliya, don daidai auna, ƙasa danshi sa ido, ta atomatik ruwa, da dai sauransu

Main fasaha nuna alama:

1. All-in-multi-aiki atomatik hoto bincike dandali, hadewa da mai hankali LED haske tushen da kuma chlorophyll fluorescence hoto kayan aiki, RGB hoto bincike kayan aiki da sauran zaɓi hoto kayan aiki kamar infrared zafi hoto, LiDAR laser dubawa, high spectrum da sauransu, ta atomatik gudanar da tsarin aiki, atomatik rarraba ajiya, atomatik online bincike da sauransu

2. Chlorophyll fluorescence imaging analysis (daidaitaccen daidaitawa):

a) 3 launi mai hankali LED motsawa haske tushen, 620nm bugun jini auna haske, farin photochemical haske da mafi girma cika haske flash, 735nm infrared haske don auna Fo 'da sauransu

b) Mai zaɓi tare da tushen haske mai shuɗi tare da matattarar 7-bit don ma'aunin fluorescence mai yawa kamar GFP imaging

c) Musamman high hankali CCD Chlorophyll fluorescence hoton firikwensin, frame mita har zuwa 50fps, ingantaccen kama Chlorophyll fluorescence transient, ƙuduri 720x560 pixels, A / D 12bit, tare da yanayin bidiyo da yanayin hoto; Zaɓi tare da high ƙuduri CCD, ƙuduri 1360x1024, frame mita 20fps, A / D 16bit

d) Yankin hoto guda ɗaya 35x35cm

e) Imaging ma'auni sigogi: Za a iya yin duhu dare duhu daidaitawa ma'auni da rana haske daidaitawa ma'auni, ma'auni sigogi ciki har da Fo, Fm, Fv, Fo’, Fm’, Fv’, Ft, Fv/Fm, Fv’/Fm’, Phi_PSII, NPQ, qN, qP, Rfd Others chlorophyll fluorescence sigogi don nazarin tsire-tsire photosynthesis inganci, dacewa, halitta da kuma non-halitta tilasta da amfanin gona juriya, juriya da sauransu

f) Fv / Fm, Kautsky induction tasiri, fluorescence kashewa bincike da sauransu cikakken atomatik ma'auni tsari (protocols) da ma'auni sigogi, kamar Fv / Fm tsari ma'auni lokaci dauki kawai 10s

g) Chlorophyll fluorescence data online bincike, ciki har da columnar zane-zane, auna sigogi zane-zane, data tebur da sauransu, tare da al'ada image raba da sauransu ayyuka, za a iya yin multi-sigogi m bincike na daban-daban lokaci sikelin (kamar rana, watanni, dukan girma kakar da sauransu)

h) ne ainihin 2D aiki tare da hoto, samun chlorophyll fluorescence sigogi ne ainihin 2D rarraba sigogi bisa ga pixel maki, kauce wa sauƙaƙe "laser induced hoto" (amfanin ne haske, ceton wutar lantarki) kawai daya-dimensional hoto (maki ko layi), ba zai iya aiki tare da 2D hoto, m muhalli dalilai (kamar iska buga buga samar da mummunan kuskure), hoto sigogi ne kawai analog sigogi (da sauri auna fluorescence da jinkirin auna fluorescence samuwa bisa ga laser bincike ba gaske mafi ƙarancin fluorescence da mafi girma fluorescence, samun sigogi "haske quantum samarwa" ne kawai analog haske quantum samarwa bukatar amfani da daidaitawa bayan sigogi don amfani da), auna sigogi guda daya (kawai samun sauri auna fluorescence da jinkirin auna fluorescence da kuma da haka lissafi analog haske quantum samarwa ko haske quantum inganci), Matsaloli kamar rashin maturity na fasaha (ba za a iya samun takardun shaida ba)

i) shine mafi yawan amfani da fasaha a duniya don binciken phenotype na shuka masu yawa

3.RGB image analysis (misali): Za a iya yin nazarin hoto na siffar shuka, launin kore, da dai sauransu, ƙuduri 5Mpx, kuma za a iya yin nazarin kididdiga ta atomatik kan adadin furanni na shuka, shinkafa, da dai sauransu, manyan sigogin auna bincike sun haɗa da:

Yankin ganye: Amfani don sa ido kan yawan ci gaba da canje-canje

2)植物紧实度/紧密度 ((Tsarin ƙarfi/Compactness. rabo tsakanin yankin da tsire-tsire na tsire-tsire ya rufe da yankin da tsire-tsire ya rufe)

3) 叶片周长 ((Leaf Perimeter: Musamman mai amfani don siffar ganye ta asali da kimantawa na fadi (haɗe tare da yankin ganye) )

4) 偏心率 ((Eccentricity: Tsarin siffar tsire-tsire, lambar scalar, eccentricity na ellipse tare da wannan lokacin na biyu kamar tsire-tsire (0...da'irar, 1...layin sashe) )

5) 叶圆度 ((Roundness: Bisa ga kimanta rabo tsakanin yankin ganye da kewayon. Yana ba da bayani game da zagaye na ganye)

6) 叶宽指数 ((Matsakaicin Ganye Width Index: Yankin ganye daidai da kwalliyar shuka (watau rage ganye zuwa sashin layi))

7)叶片细长度SOL (Ƙananan Ganye)

8)植物圆直径(Diamita na zagaye. Diamita na zagaye tare da yanki ɗaya kamar shuka)

9) 凸包面积 ((Convex Hull Area. Amfani don kimantawa na compactness)

10) 植物质心 ((Centroid. Cibiyar matsayin tsire-tsire (musamman mai amfani don kimantawa na eccentricity))

11) Ƙididdigar ƙididdiga (Flattening index)

12) Ƙarin girma na dangantaka (Relative growth rate)

13) Index na kore da nazarin darajar (Dark Green, Healthy Green, Light Green, da dai sauransu)

Rarraba launi don kimantawa na motsa jiki na shuka

15) Sauran halaye da launi grading dynamic analysis

4.3D Laser binciken bincike (zaɓi): Ana amfani da tsire-tsire tsarin phenotype bincike, ta atomatik lissafi tsire-tsire tsarin, biomass, lambar ganye, ganye yankin, ganye karkata kusurwa, tsire-tsire tsayi da sauran daban-daban morphological tsarin sigogi

5. Infrared thermal imaging bincike (zaɓi): Focal Plane Array Microthermal Radiometer, ƙuduri 640 × 480 pixels, band 7.5-13μm, zafin jiki kewayon -20 – 120 ℃, ƙuduri <0.05 ℃ @ 30 ℃ / 50mK, imaging yankin 35x35cm, don imaging tsire-tsire na coronary zafin jiki rarraba a cikin haske radiation yanayi, da kuma nazarin tsire-tsire na aeroporous conductivity dynamics, bushewa tilasta da kuma juriya bushewa ikon kimantawa da dai sauransu, kyakkyawan zafi iya sa tsire-tsire jure tsawon lokaci high haske radiation ko low ruwa yanayi (bushewa)

6. High spectrum imaging analysis (zaɓi): wavelength kewayon 380-1000nm, spectrum bands (bands) 675, za a iya daukar hoto da kuma nazarin daidaitaccen ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar ƙididdigar

7. Yankin yanar gizo na yanar gizo: Yankin 12m na dandamali, dandamali na hoto mai amfani da dama yana iya bincika hoton hoto ta atomatik a kan dandamali na yanar gizo, yana iya bincika samfurin samfurin har zuwa 10m, kowane yankin hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton Tsawon dandamali na wayar hannu 2.5m, dandamali mai amfani da hoto mai yawa yana daidaitawa don daidaitawa da nazarin hoton amfani da gona daban-daban; Mobile dandamali 4 roba ƙafafun za a iya gudanar da atomatik ta hanyar sarrafa tsarin da kuma atomatik duba hoto, kuma sauki a kan gaba daya ƙasa motsi, juyawa, da dai sauransu, ga samfurin 75x20m, mobile dandamali za a iya ɗaukar multi-aiki hoto dandamali a lokaci guda kammala 75x10m samfurin bands, sa'an nan kuma hannu juyawa bayan atomatik kammala wani rabin 75x10m hoto nazarin; Sanye da GPS tsarin daidaito har zuwa 2cm, ta atomatik rikodin ma'auni data, wuri, lokaci, da dai sauransu ta hanyar software, za a iya samar da wutar lantarki ta hanyar injinar dizal don motsa dukan dandamali

8. Za a iya zaɓi tare da yanayin ma'auni firikwensin cibiyar sadarwa, atomatik sa ido rikodin PAR, yanayin CO2 matattarar, iska zafi da zafi, ruwan sama da ƙasa zafi da sauransu.

9. Tsarin sarrafawa da tsarin nazarin tattara bayanai (babban dandalin bayanai na phenotype):

1) Mai amfani-friendly graphical dubawa

2) GPS location aiki zai iya yin sararin samaniya rarraba bayanai da kuma lokaci sararin samaniya rarraba tsari analysis

3) An gina nau'ikan manyan Protocols, tare da ayyana mai amfani, shirye-shiryen auna ta atomatik (protocols), daidai da tsarin saitin mai amfani don kammala duk gwajin ta atomatik. Data sakamakon ta atomatik adana da kuma bincike, da kuma bincike data sakamakon za a iya nuna ta atomatik a matsayin m curves

4) Tsarin sarrafa bayanan MySQL, wanda zai iya sarrafa manyan bayanan tare da miliyoyin bayanai, yana tallafawa injunan ajiya da yawa, ana adana bayanan da suka shafi su ta atomatik a cikin tebur daban-daban a cikin bayanan

5) Za a iya yin duk ma'auni tare da tsoho shirin, kuma za a iya ƙirƙirar al'ada aiki tsari ta hanyar ci gaba kayan aiki, ko hannu aiki LED haske tushen kunna ko kashe, RGB scan hoto, chlorophyll fluorescence hoto da sauransu

6) Shirye-shiryen gwaji (Protocols) tare da maɓallin farawa, maɓallin ƙarshe, maɓallin dakatarwa

7) Tsarin za a iya sarrafa nesa ta hanyar Intanet mara waya, yana ba masu amfani damar samun damar nesa ta hanyar Intanet, sarrafa bayanai, saukewa da canza ƙirar gwaji, tare da aikin ƙididdigar mai amfani don hana wasu mutanen da ba su yi aiki da kyau don tasirin gwaji

Asalin: Turai PSI

Aikace-aikace:

Aikace-aikacen FluorCamChlorophyll fluorescence fasaha, don in situ daban-daban yanayi dogon lokaci sa ido kan daji tsire-tsire, yayin da sa ido kan tsire-tsire photosynthesis (CO2 assimilation) A, sakamakon duba hoton da ke ƙasa. FluorCam Chlorophyll Fluorescence Technology yana amfani da fasahar motsawa mai haske, fasahar CCD mai haske (samfurin samfurin har zuwa 50 sau a kowane dakika) da kuma tushen hasken LED mai hankali, wanda zai iya zama babban yanki (daidaitacce tare da yankin hoto na 35x35cm a kowane frame) Tsire-tsire / amfanin gona image analysis, a cikin daji za a iya gudanar da biyu a dare a karkashin duhu daidaitawa yanayi na chlorophyll fluorescence image analysis, kuma za a iya gudanar da chlorophyll fluorescence image analysis a karkashin yanayin muhalli haske daidaitawa yanayi, fiye da sauki laser haifar da chlorophyll fluorescence ma'auni (ta hanyar wani haske ko layi mai launi daya motsa haske tushen motsa chlorophyll fluorescence da kuma auna, amfanin shi ne ceton wutar lantarki da kuma iya zama mafi haske) idan aka kwatanta da akwai da yawa ayyuka fa'idodi, ba kawai ma'auni sigogi da yawa, za a iya gudanar da daban-daban na chlorophyll fluorescence gwaji tsari image ma'auni analysis, har ma da daya biyu-dimensional imaging (ainihin image analysis) kauce wa maki ko layi motsa haske bincike

Attachments: Sauran filin phenotype imaging nazarin tsarin:

1)PhenoUASdrone babban gudu Daida amfanin gona phenotype bincike dandali

2)FluorCamDaji mobile chlorophyll fluorescence da RGB hoto nazarin tsarin

3)FluorCamSamfurin dubawa na chlorophyll fluorescence tare da tsarin nazarin hoto na RGB (na zaɓi tare da hoton zafi na infrared)