Babban dandalin hoto mai ƙwarewa

ECLIPSE Ti2 yana samun 25mm Vision (FOV) wanda ba a taɓa gani ba, yana canza yadda kake kallon. Tare da babban hangen nesa mai ban mamaki, Ti2 yana iya amfani da yankin firikwensin manyan kyamarorin CMOS masu manufa kamar yadda yake so, kuma yana haɓaka yawan tattara bayanai sosai.

An tsara shi don tsarin hoto mai ƙuduri sosai, Ti2 mai ɗaukar hoto yana aiki sosai tare da kwanciyar hankali, ba tare da canzawa ba, yayin da fasalin ƙaddamar da kayan aikinsa na musamman ke ba da sauƙin sarrafa gwaje-gwajen hoto masu sauri. Ti2 musamman mai hankali kayan tattara ciki firikwensin bayanai, jagoranci masu amfani da cikakken image tsari, hana kuskure aiki. Bugu da ƙari, a lokacin tattara bayanai, za a yi rikodin yanayin daban-daban na'urorin firikwensin ta atomatik, wanda a ƙarshe zai sami hoto mai inganci don inganta sake dubawa na bayanai.

Tare da NIS-Elements, software mai karfi na Nikon na tattara hoto da bincike, Ti2 ya cancanci zama jagora a cikin ƙirar hoto.

|Babban hangen nesa

Yayin da yanayin bincike ke ci gaba zuwa babban, tsarin tsarin, kasuwar tana ƙaruwa don saurin tattara bayanai da ƙarin iyawa. Ci gaban manyan na'urorin firikwensin kyamara da ingantaccen damar sarrafa bayanai na kwamfuta sun haifar da irin wannan yanayin bincike. Tare da hangen nesa na 25mm da ba a taɓa gani ba, Ti2 yana ba da matakin ƙididdiga mafi girma wanda ke ba masu bincike damar haɓaka matsayin manyan masu ganowa, tabbatar da cewa dandalin hoto na asali ya dace da buƙatun nan gaba yayin da fasahar kyamara ke ci gaba da haɓaka cikin sauri.

Microtubular launi na neuron (Alexa Fluor 488); Yi harbi tare da CFI Plan Apo lambda 60x abubuwa da kyamarar DS-Qi2. Hoton da ke sama shine hangen nesa na gargajiya, hoton da ke ƙasa shine sabon hangen nesa na Ti2.
Hoton ta Josh Rappoport, Cibiyar Hoton Nikon ta Arewa maso Yammacin Jami'ar;
Jami'ar Arewa maso Yammacin S. Kemal, B. Wang da R. Vassar sun ba da samfurin.

|Hasken filin haske na babban hangen nesa

High ikon LED samar da haske haske a cikin babban hangen nesa na Ti2, tabbatar da bayyane, daidai sakamako a karkashin tsananin bukatun kamar babban girma bambanci tsangwama (DIC). Tare da zane na ruwan tabarau mai rikitarwa, Ti2 yana iya samar da haske mai haske daga gefe ɗaya zuwa gefe ɗaya. Wannan yana da amfani sosai ga ƙididdigar hoto mai sauri da haɗin hoto mai girma.

High ikon LED haske

Gina-in ruwan tabarau mai rikitarwa

Mun tsara kayan aiki na musamman don hoton hoton hoton hoto. Yana sanye da ruwan tabarau mai haske mai haske mai kwartas kuma yana ba da babban ƙimar watsawa a cikin faɗin spectrum ciki har da ultraviolet. Babban size fluorescent tace tare da wuya rufi iya samar da babban hangen nesa image yayin da tabbatar da high sigina amo rabo.

Babban hangen nesa faduwa fluorescent lighter

Babban size fluorescent tace

|Kulawa da hanya na haske tare da babban diameter

Kula da fadada diamita na hanyar haske, yana ba da damar tashar jirgin ruwa ta yi yawan filin gani na 25. Babban hangen nesa da aka samu yana iya ɗaukar hoto na yanki kimanin sau biyu na ruwan tabarau na gargajiya, yana ba masu amfani damar yin amfani da mafi kyawun aikin manyan na'urorin firikwensin kama da masu gano CMOS.

Widened lasisi

Babban tashar jirgin ruwa tare da yawan filin gani na 25

|Abubuwa don hoton babban filin gani

Abubuwan da ke da kyakkyawan filin hoto yana tabbatar da ingancin hoto daga gefe ɗaya zuwa gefe ɗaya. Cikakken amfani da mafi girman damar abubuwan OFN25 na iya hanzarta tsarin tattara bayanai sosai.

|Kamara don tattara bayanai mai yawa

Babban ƙwarewar kyamarar launi ɗaya DS-Qi2 da kyamarar launi mai sauri DS-Ri2 yana da girman 36.0 x 23.9 mm da na'urar firikwensin CMOS mai pixel 1625, wanda zai iya samun mafi kyawun aiki daga babban hangen nesa na Ti2 25mm.

D-SLR kamara fasaha ingantacce don microscope

DS-Qi2

DS-Ri2

|Kyakkyawan Nikon na'urorin gani

Nikon ta high daidaito CFI60 Unlimited nesa optical na'urorin da aka tsara don daban-daban rikitarwa lura hanyoyin, da kuma m amincewa da masu bincike yabo tare da kyakkyawan optical aiki da kuma m.

|Yankan Toe bambanci

Nikon na musamman yankan yatsan yatsan bambancin abubuwa amfani da zaɓaɓɓun amplitude tace, iya muhimmanci haɓaka bambanci da rage halo illusion, don samar da m HD hotuna.

Yankan yatsan yatsan mataki board haɗin APC abubuwan

Kwayoyin BSC-1 da aka ɗauka ta amfani da CFI S Plan Fluor ELWD ADM 40xC

|Bambancin waje (Ti2-E)

Electric waje bambanci tsarin ta hanyar guje wa amfani da bambanci abubuwa, mai amfani ya hada bambanci tare da faduwa fluorescence imaging, ba tare da tasiri da ingancin fluorescence. Alal misali, ana iya amfani da abubuwan ruwa tare da babban ƙididdigar ƙididdiga (NA) don yin hoton bambanci. Ta hanyar wannan tsarin bambanci na waje, masu amfani za su iya haɗa bambanci da sauran yanayin hoto da sauƙi, gami da hoto mai rauni kamar TIRF da haske.

Dropping fluorescence da waje bambanci image:
Kwayoyin PTK-1 da aka yi alama da GFP-alpha microtubulin, hoton da aka ɗauka ta amfani da CFI Apo TIRF 100x Oil Objective ta Wadsworth Center PhD Scientific Research VI / Farfesa Alexey Khodjakov

|DIC (bambancin tsangwama)

Na'urorin gani na Nikon DIC masu girmamawa suna ba da hotuna masu daidaito, masu kyau, masu ƙuduri da bambanci a kowane ƙarin girma. An tsara DIC Prism musamman don kowane abu, yana ba da damar samar da mafi ingancin hoton DIC ga kowane samfurin.

Shigar da DIC prism daidai da daban-daban abubuwa a kan abu turntable

Bambanci tsangwama bambanci (DIC) da kuma faduwa fluorescent hotuna:
Hoton neuron mai girman 25mm (DAPI, Alexa Fluor 488, Rhodamine-Phalloidin); Hotuna tare da CFI Plan Apo lambda 60x abubuwa da kyamarar DS-Qi2 Josh Rappoport, Cibiyar Hoton Nikon ta Arewa maso Yammacin Jami'ar; Jami'ar Arewa maso Yammacin S. Kemal, B. Wang da R. Vassar sun ba da samfurin.

|NAMC (Nikon Advanced Modulation bambanci)

Wannan shine fasahar hoto mai bambanci mai dacewa da allon filastik. Yana aiki a kan samfuran da ba su da launi, kamar ƙwayoyin egg. NAMC yana ba da hotuna masu girma uku ta hanyar sakamakon hasashen. Mai amfani zai iya daidaita bambancin shugabanci da sauƙi ga kowane samfurin.

NAMC samar da kwaikwayon hoton 3D ta hanyar sakamakon hasashen

Nikon Advanced Modulation bambanci (NAMC) Hoton:
Kwayar linzami, hoto ta amfani da CFI S Plan Fluor ELWD NAMC 20x abu

|Auto gyara zobe (Ti2-E)

Canje-canje a kan samfurin kauri, murfin slide kauri, samfurin refractive rarraba da kuma zafin jiki duk na iya haifar da ball bambanci da image karkatarwa. Mafi ingancin abubuwa sau da yawa suna daidaitawa da zoben daidaitawa don biyan waɗannan canje-canje. Daidai daidaitawar zoben gyara shine mabuɗin samun hoto mai ƙuduri da bambanci mai girma. Wannan sabon madaidaiciyar gyara ta atomatik yana taimaka wa masu amfani su daidaita su zuwa mafi kyawun matsayi a kowane lokaci ta hanyar amfani da harmonic drive da algorithms na gyara ta atomatik don samun mafi kyawun aikin abu.

Harmonic drive tsari don daidai sarrafa gyara zobe daidaitawa

Hotuna masu ƙuduri (DNA PAINT):
Kwayoyin CV-1 da ke bayyana alfa microtubulin (kore) da TOMM-20 (yellow), an harba su ta amfani da CFI Apo TIRF 100x Oil Objective.

|Falling fluorescence

L jerin abubuwan da aka yi amfani da Nikon patented Nano Crystal Coat fasahar, sa shi ne m zaɓi ga high bukatun, rauni sigina, multi-tashar fluorescence hoto. Saboda duk waɗannan aikace-aikace suna buƙatar tsarin don kiyaye ingancin watsawa da daidaitawa a cikin kewayon tsawon raƙuman ruwa. Sabbin matattarar fluorescence suna da mafi girman watsawa na fluorescence kuma suna da fasahohin kawar da haske kamar Noise Terminator. Tare da irin wannan matattarar fluorescence, L jerin abubuwa sun tabbatar da iyawarsu a yankunan kallon fluorescence mai rauni, gami da hoton kwayoyin halitta guda ɗaya da aikace-aikacen tushen haske mai sanyi.

Harmonic drive tsari don daidai sarrafa gyara zobe daidaitawa

Hoton sanyi:
Bayyana BRET-tushen calcium nuna alama furotin, nano calcium cage Hela kwayoyin.
Dr. Takeharu Nagai daga Cibiyar Kimiyya da Masana'antu ta Jami'ar Osaka, Japan, ya ba da samfurin

|Perfect mayar da hankali

Ko da ƙananan canje-canje na zafin jiki da ƙananan rawar jiki na yanayin hoto na iya shafar kwanciyar hankali sosai. Ti2 yana amfani da matakai masu tsayayye da masu motsi don kawar da sauyawar fuska, don haka yana iya nuna nano da microscopic hotuna a cikin gwajin dogon lokaci.

|Injin sake tsarawa, samun super high kwanciyar hankali (Ti2-E)

Don inganta kwanciyar hankali, an sake tsara tsarin sarrafa kansa na lantarki Z axis da cikakken tsarin mayar da hankali (PFS). Sabuwar tsarin Z-axis mai mayar da hankali yana da ƙananan girma kuma yana kusa da abubuwan da ke juyawa don rage rawar jiki. Ko da a cikin fadada (biyu layers optical) saiti, shi ne kusa da abu juyawa kwamfutar, tabbatar da kyakkyawan kwanciyar hankali a duk aikace-aikace.

Ko da a cikin fadada saiti, da high kwanciyar hankali Z axis mayar da hankali tsarin kusa da abu turntable

An raba sashin mai ganowa na Perfect Focus System (PFS) daga kwamfutar juyawa don rage nauyin inji a kan kwamfutar juyawa. Wannan sabon zane kuma yana rage watsa zafi, yana taimakawa wajen ƙirƙirar yanayin hoto mai kwanciyar hankali. Saboda haka, amfani da wutar lantarki na motar Z-axis ya rage. Wadannan sake tsarawa a kan inji suna ba da babban kwanciyar hankali ga dandalin hoto, yana sa ya dace da aikace-aikacen hoto na molecule guda ɗaya da ƙuduri mai girma.

|Tsarin sarrafa kansa na sabon ƙarni tare da PFS: Cikakken (Ti2-E)

Cikakken tsarin mayar da hankali na ƙarni na ƙarshe (PFS) yana iya gyara ta atomatik canje-canje na zafin jiki da motsin jiki na inji (sau da yawa ana gabatar da irin wannan tsangwama lokacin ƙara reagents zuwa samfurin da hoto mai yawa).

PFS yana ganowa a ainihin lokacin kuma yana bin diddigin wurin da farashin tunani yake (misali farashin rufi lokacin amfani da abubuwan ruwan ruwa) don kiyaye farashin mai mayar da hankali. Fasahar musamman ta optical compensation tana ba masu amfani damar kiyaye fuskar a kowane matsayi na dangantaka na tunani. Mai amfani zai iya mai da hankali kai tsaye kan jirgin sama da ake so, sa'an nan kuma kunna PFS. PFS yana aiki ta atomatik ta hanyar ginin masu tsara layi da kuma hanyoyin amsawa masu sauri kuma yana kiyaye mai mayar da hankali don samar da hotuna masu aminci har ma a cikin dogon lokaci, hadaddun ayyukan hoto.

PFS ya dace da aikace-aikace daban-daban, daga gwaje-gwaje na yau da kullun a kan kwandon petri na filastik zuwa daukar hoto na kwayoyin halitta guda ɗaya da kuma daukar hoto na photon da yawa. Har ila yau, yana dacewa da tsawon raƙuman ruwa daban-daban, daga UV zuwa infrared, wanda ke nufin cewa ana iya amfani da shi don aikace-aikacen photon da photon.

|Taimakawa Wizard

Ba a ƙara bukatar tunawa da rikitarwa microscope daidaitawa da kuma aiki matakai. Ti2 zai iya haɗa bayanai daga na'urorin firikwensin don jagorantar ku a cikin waɗannan matakai don kauce wa kuskuren aikin mutum, yana ba masu bincike damar mai da hankali kan bayanai.

|Cikakken nuna yanayin microscope (Ti2-E / A)

A jerin gina-in firikwensin gano da kuma watsa aiki yanayin bayanai na daban-daban sassa na microscope. Lokacin da kake amfani da kwamfutarka don samun hoto, duk bayanan yanayin za a rubuta su a cikin metadata don tabbatar da cewa za ka iya kiran yanayin karɓar da sauƙi da / ko bincika kuskuren saitunan. Bugu da ƙari, gina-in kyamara damar mai amfani duba baya mayar da hankali jirgin sama, sauki daidaita bambanci zoben da kuma DIC ta dimming gicciye. Hakanan yana ba da tsarin daidaitawar laser mai aminci don aikace-aikace kamar TIRF.

Ginin-firikwensin gano yanayin microscope sassa

Za a iya ganin yanayin microscope ta hanyar kwamfutar hannu ko ta hanyar alamar yanayin microscope a gaban kwamfutar hannu. Wannan yana ba da damar binciken yanayin a cikin ɗakin duhu.

Yanayin nuna alama

|Aiki Mataki Wizard (Ti2-E / A)

Ti2 ta taimako wizard aiki samar da m mataki-mataki jagora ga microscope aiki. Ana iya duba wannan fasalin a kan kwamfutar hannu ko kwamfuta, kuma yana haɗuwa da ainihin bayanan lokaci daga na'urorin firikwensin da aka gina da kyamarar ciki. Taimakon Wizard zai iya taimaka wa masu amfani su kammala gwajin saiti da warware matsala.

|Gano kuskure ta atomatik (Ti2-E / A)

Ta hanyar yanayin dubawa, mai amfani zai iya tabbatar da cewa duk abubuwan da suka dace da hanyar dubawa da aka zaɓa suna a wuri a kan kwamfutar hannu ko kwamfutar. Lokacin da hanyar lura da aka zaɓa ta kasa aiwatar, wannan yanayin bincike na iya rage lokaci da ƙoƙari da ake buƙata don magance matsaloli. Wannan aiki yana da amfani musamman ga yanayin da masu amfani da yawa ke amfani da shi, saboda kowane mai amfani zai iya canza saitunan microscope. Masu amfani kuma za su iya pre-shirya al'ada bincike shirye-shirye.

Nuna abubuwan da ba daidai ba

|intuitive aiki

An sake tsara Ti2 gaba daya - sabon zaɓi da tsari daga tsarin jiki gaba daya zuwa kowane maɓallin da sauyawa - yana kawo ƙwarewar mai amfani mai kyau. Ana iya amfani da waɗannan sarrafawa cikin sauƙi har ma a cikin ɗakunan duhu (mafi yawan gwaje-gwaje ana gudanarwa a cikin ɗakunan duhu). Ti2 samar da wani intuitive da kuma sauki mai amfani dubawa, tabbatar da masu bincike iya mayar da hankali kan bayanai maimakon microscope aiki da kuma iko.

|Tsarin da aka tsara don sarrafa microscope (Ti2-E / A)

Dukkanin maɓallin da kayan da aka canza sun dogara ne akan nau'in hasken da aka sarrafa. Button don sarrafa kula da watsawa yana gefen hagu na microscope, yayin da button don sarrafa kula da faduwar fluorescence ke gefen dama. Buttons don sarrafa yau da kullun aiki a kan gaban panel. Wannan tsarin rarrabawa yana sauƙaƙe ƙwaƙwalwar ajiya kuma yana da amfani musamman lokacin aiki da microscope a cikin ɗakin duhu.

An haɗa sauyawa mai sauyawa a cikin zane na microscope don sarrafa na'urori kamar na'urorin juyawa na matattarar fluorescent da na'urorin juyawa na abubuwan da aka yi amfani da su. Wadannan sauyawa kwaikwayon hannu juya da na'urar da aka ambata a sama da kuma samun intuitive iko. Sauran ayyuka kuma za a iya haɗawa a cikin waɗannan canje-canje masu sauyawa don tabbatar da cewa canje-canje guda ɗaya zai iya aiki da na'urori da yawa masu dacewa. Misali, juyawa da juyawa na fluorescent tace juyawa ba kawai zai iya juyawa da juyawa ba, har ma za a iya canza fluorescent shutter yayin da mai amfani ya latsa canzawa. Bugu da ƙari, ana iya shirya waɗannan canje-canje don aiki da matattarar ƙaddamarwa da na'urorin bambanci na waje.

<unk> Shirye-shiryen maɓallin aiki (Ti2-E / A)

An tsara maɓallin gajeren hanya don sauƙaƙe mai amfani da tsara ayyuka. Masu amfani za su iya zaɓar daga fiye da ayyuka 100, gami da sarrafa na'urorin lantarki kamar shutters, har ma da fitarwa guda ɗaya zuwa na'urorin waje ta hanyar tashar jiragen ruwa na I / O da ake amfani da su don karɓar trigger. Hakanan za a iya ƙayyade yanayin aiki ga waɗannan maɓallin, don haka za a iya canza hanyar kallo a kowane lokaci ta hanyar adana daban-daban na'urorin lantarki.

Tuning Acceleration button da Perfect mayar da hankali tsarin (PFS) kunna button kusa da daidaitawa knob. Dangane da siffofi daban-daban, za a iya gane maɓallin aiki daban-daban da sauƙi ta hanyar taɓawa. Saurin mayar da hankali yana daidaitawa ta atomatik bisa ga abubuwan da ake amfani da su a halin yanzu. Wannan yana ba masu amfani damar samun ingantaccen saurin mayar da hankali a ƙarƙashin abubuwa daban-daban, yana sa aikin microscope ya zama mai sauƙi.

|Amfani da sarrafa sanduna da kwamfutar hannu (Ti2-E)

Ti2 sarrafawa ba kawai sarrafa motsi na teburin ɗaukar kaya ba, har ma yana sarrafa yawancin ayyukan lantarki na microscope, gami da yanayin kunnawa na cikakken tsarin mayar da hankali (PFS). Yana iya nuna yanayin XYZ coordinates da kuma microscope sassa, sosai sauki mai amfani da nesa sarrafawa. Masu amfani kuma za su iya sarrafa aikin lantarki na Ti2 daga kwamfutar hannu da aka haɗa da microscope ta hanyar Wi-Fi, don cimma cikakken kwarewar aikin gani na microscope.